Triggerable Multivalent Glyconanoparticles for Probing Carbohydrate–Carbohydrate Interactions

Glyconanoparticles and their interactions with lectins

Probing Low Affinity and Multivalent Interactions with Surface Plasmon Resonance: Ligands for Concanavalin A

The affinities of the carbohydrate-binding protein concanavalin A (Con A) for monoand multivalent ligands were measured by surface plasmon resonance (SPR) detection. Assessing protein-carbohydrate affinities is typically difficult due to weak affinities observed and the complications that arise from the importance of multivalency in these interactions. We describe a convenient method to rapidly...

Glyconanoparticles for colorimetric bioassays.

Carbohydrate molecules are involved in many of the cellular processes that are important for life. By combining the specific analyte targeting of carbohydrates with the multivalent structure and change of solution colour as a consequence of plasmonic interactions with the aggregation of metal nanoparticles, glyconanoparticles have been used extensively for the development of bioanalytical assay...

Probing multivalent carbohydrate-lectin interactions by an enzyme-linked lectin assay employing covalently immobilized carbohydrates.

We report here the synthesis of a series of mono- to trivalent N-acetylglucosamine (GlcNAc) derivatives as ligands for the plant lectin wheat germ agglutinin (WGA). Their WGA binding potencies were determined by an established enzyme-linked lectin assay (ELLA) employing microtiter plates with non-covalently immobilized porcine stomach mucin (PSM) as reference ligand and an ELLA with a new GlcNA...

Stabilizing Protein-Protein-Interactions with Multivalent Ligands

Huntington’s disorder (HD) is one of the neurodegenerative disorders in which the polyglutamine (polyQ) tract of the protein huntingtin (htt) is found to be expanded. First exon of this mutant htt protein is found in the inclusions in the brain of the patient suffering from HD, which is the pathological hallmark of the disease1. Exon-1 of htt comprises of 17 amino acid long N-terminal helix (Nt...